Dr. Pauline Nys
Member

Areas of expertise include:
Physical and Chemical Kinetics and Thermodynamic; Absorption Processes of Organic compounds; Processes of Enzyme Engineering for Antibiotic production; Processes of Enzyme isolation, purification, modification and immobilization.

Research experience:
2003-up to date
Eastern Biotech Inc., New York, USA - Scientific Advisor

2000-2003
Piselli Consulting S.L., Barcelona Spain – Scientific Consultant

1962-2000
National Research Center for Antibiotics, Moscow, Russia - the major research center of pharmaceutical industry in the former Soviet Union.

Chief of the Lab. of Enzyme Engineering, Chief Research Scientist, Senior Research Scientist, Postgraduate Student responsible for:

-Elaboration the scientific basis for design and optimization of enzymatic processes and development of the original processes for production of antibacterial, anticancer, antifungal antibiotics and the key compounds required for their synthesis. The developed methods for beta lactam compounds were utilized by industry.

-Formulation the general principles of design the highly efficient biocatalyst for the synthesis and transformation of labile compounds on the base of immobilized enzymes and cells. Development of the novel industrial processes for biocatalyst production.

Education:
1990--National Research Center for Antibiotics, Moscow, Russia, Professor in Biotechnology

1984--National Research Center for Antibiotics, Moscow, Russia. Dr.of Sci. in Bioorganic Chemistry 1964-1968
Karpov Physico-Chemical Institute, Moscow, Russia. Ph. D. in Physical Chemistry. 1955-1960
University of Chemical Technology, Kazan, Russia. MS in Inorganic Chemistry.

Publications:
Over 250 scientific papers in Russian and International journals, significant part of which published in the US. Over 50 Author's Certificates (USSR patents), half of which have found practical application in industry.

Annex
1. "Comparison of the synthetic and hydrolytic activity of immobilized cephalexin-synthetase" – Biocatalysis, 1994, 11, 315-320.
2. "Role of biocatalysis in development and improvement of processes for production of betalactam antibiotics in Russia" - Antibiotiki i Khimioterapiya (Russia), 1994, 39, No 8, 3-14.
3. "Mathematical modelling and optimization of industrial processes for antibiotic production" – Preprints of the 6th Int. Conference on Computer Applications in Biotechnology, Garmisch-Partenkirchen, Germany, 1995, 268-271.
4. "Mathematical model of acyl transfer synthesis of semisynthetic cephalosporins"- IMACS/IFAC First Symp. on Mathematical modelling and simulation in agriculture and bio-industries, Brussel, Belgium, 1995,.1V, IV.B3-1 - IV.B3-5.
5. "Enzymatic synthesis of betalactam antibiotics. I. Cefazolin" - Antibiotiki i Khemioterapiya (Russia), 1999, No 5, 12-16
6. "Enzymatic synthesis of betalactam antibiotics. II. Aminocephalosporins"- Antibiotiki i Khemioterapiya (Russia), 1999, No 8, 6-11.
7. Analysis and prognosis of science in the area of antibiotic production in Russia". Pharmaceutical bulletin, 2000, ? 17, ?.18-19.
8. "Methodological approach to development of enzymatic technologies for semisynthetic antibiotic production"- Appl. Biochem. Biotechnol. 2000, Part A Enzyme Eng Biotechnol 88, 221-229.
9. "Enzymatic transformation of betalactam antibiotics. Trends of development and approaches to practical implementation." Moscow University Chemistry Bulletin, 2000, Special Issue, p.41-44
10. "Beta-lactam compounds. Interrelations of the structure and biological activity" Antibiotics and Chemotherapy, 2000, ? 11, 36-42
11. "Novel beta-lactam structures. The construction problems"- Antibiotics and Chemotherapy, 2002, ? 2, 29-37.
12. "Kinetic and thermodynamic approach to design the processes for enzymatic synthesis of betalactams" - Biocatalysis and Biotransformation, 2003, V. 20, N 1, pp.35-41.
13. "Combinatorial enzymology. Synthesis of novel betalactam libraries". Moscow University Chemistry Bulletin, 2003, Special Issue, p.137-141

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